human hcc hepg2 cells  (ATCC)

Journal: Scientific Reports

Article Title: Development of an innovative nanopolymer-lncRNA-SRHC complex as therapeutic modalities for targeted hepatocellular carcinoma therapy

doi: 10.1038/s41598-026-51340-1

Figure Lengend Snippet: Evaluation of cytotoxicity profiles of SRHC nanoparticles. The dose-response curve illustrates the relationship between the cytotoxicity percentage of SRHC (blue) and conjugated NPs (Polymer-SRHC) (red) on HepG2 cells and the concentration in nmol. The IC 50 values of SRHC and conjugated NPs (Polymer-SRHC) were determined from the curve to be 98.8 nmol and 95.24, respectively.

Article Snippet: Human HCC HepG2 cells (ATCC HB-8065) were cultured in 25 cm 2 culture flasks.

Techniques: Polymer, Concentration Assay

Journal: Translational Oncology

Article Title: Integrating spatial and single-cell transcriptomics via machine learning to characterize efferocytosis in hepatocellular carcinoma prognosis and immunotherapy

doi: 10.1016/j.tranon.2026.102801

Figure Lengend Snippet: Identification and multi-level validation of TPI1 as a pivotal prognostic driver. (A) Lollipop chart showing the selection frequency of feature genes across 101 machine learning models, identifying TPI1 as a high-frequency core gene. (B) Univariate Cox regression analysis of candidate genes; TPI1 exhibited the most substantial Hazard Ratio (HR), characterizing it as a preeminent risk factor. (C) Expression profiling of TPI1 across malignant versus paracancerous tissues within the TCGA-LIHC discovery cohort (upper) and GSE14520 validation cohort (lower). (D) Kaplan-Meier overall survival curves comparing patients with high and low TPI1 expression in the TCGA-LIHC (top) and GSE14520 (bottom) cohorts. (E) Relative mRNA expression levels of TPI1 in the immortalized human hepatocyte line (MIHA) and HCC cell lines (Huh7, SMMC-7721) determined by RT-qPCR. (F) Representative Western blot images (left) and densitometric quantification (right) of TPI1 protein levels in MIHA, Huh7, and SMMC-7721 cells. GAPDH served as the internal loading control. Data are expressed as mean ± SD. ** P < 0.01, *** P < 0.001.

Article Snippet: The human HCC cell lines (Huh7 and SMMC-7721) and the immortalized human hepatocyte line MIHA were procured from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Biomarker Discovery, Selection, Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: CytoJournal

Article Title: SOX2 promotes the progression of hepatocellular carcinoma by targeting FOXJ3 and activating the PI3K/AKT pathway to regulate autophagy and mitophagy

doi: 10.25259/Cytojournal_164_2025

Figure Lengend Snippet: SOX2 is highly expressed in hepatocellular carcinoma (HCC) cell lines. (a-c) qRT-PCR and Western blot analysis of SOX2 mRNA and protein expression levels in normal liver epithelial (THLE2) and HCC (Huh7) cell lines. (d-f) qRT-PCR and Western blot validation of transfection efficiency in Huh7 cells transfected with shRNA targeting SOX2 (shSOX2) or SOX2 overexpression plasmids. n = 6; ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, qRT-PCR: Quantitative reverse transcription polymerase chain reaction. mRNA: messenger RNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh-NC: shRNA-negative control; Oe-NC: overexpression plasmid negative control.

Article Snippet: Human normal liver epithelial cells (THLE-2, CL-0833) and HCC cells (Huh7, CL-0120) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Biomarker Discovery, Transfection, shRNA, Over Expression, Reverse Transcription, Polymerase Chain Reaction, Negative Control, Plasmid Preparation

Journal: CytoJournal

Article Title: SOX2 promotes the progression of hepatocellular carcinoma by targeting FOXJ3 and activating the PI3K/AKT pathway to regulate autophagy and mitophagy

doi: 10.25259/Cytojournal_164_2025

Figure Lengend Snippet: SOX2 promotes the growth of HCC cells. (a and b) EdU staining assessing the effects of SOX2 overexpression or knockdown on Huh7 cell proliferation. (a) Magnification = ×100, Scale bar: 100 μm. (c-f) Transwell assays evaluating the effect of SOX2 on Huh7 cell motility. (c and e) Magnification = ×200, Scale bar: 50 μm. (g and h) Flow cytometry analysis of SOX2 effects on apoptosis in Huh7 cells (PI and Annexin V-FITC stain). n = 6; ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, HCC: Hepatocellular carcinoma. sh-NC: shRNA-negative control; Oe-NC: Overexpression plasmid negative control; ECD-A: Energy Coupled Dye -Area; FITC-A: Fluorescein isothiocyanate -Area.

Article Snippet: Human normal liver epithelial cells (THLE-2, CL-0833) and HCC cells (Huh7, CL-0120) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Staining, Over Expression, Knockdown, Flow Cytometry, shRNA, Negative Control, Plasmid Preparation

Journal: CytoJournal

Article Title: SOX2 promotes the progression of hepatocellular carcinoma by targeting FOXJ3 and activating the PI3K/AKT pathway to regulate autophagy and mitophagy

doi: 10.25259/Cytojournal_164_2025

Figure Lengend Snippet: SOX2 induces autophagy and mitophagy in HCC cells. (a-c) Western blot analysis of the effect of SOX2 on the expression levels of autophagy-related proteins LC3II, LC3I, and p62. (d) Transmission electron microscopic observation of autophagosome formation in HCC cells following SOX2 modulation. (e-g) Western blot analysis of the effect of SOX2 on mitophagy-related proteins PINK1, phosphorylated COX4 (p-COX4), and COX4. (h) Immunofluorescence analysis of the colocalization between mitochondria and lysosomes upon SOX2 expression. (i) Relative intensity of overlapping fluorescence signals of mitochondria and lysosomes. (d) and (h) magnification = ×200, Scale bar: 50 μm. n = 6; ✶ P <0.05, ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, HCC: Hepatocellular carcinoma. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh-NC: shRNA-negative control; Oe-NC: overexpression plasmid negative control.

Article Snippet: Human normal liver epithelial cells (THLE-2, CL-0833) and HCC cells (Huh7, CL-0120) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Western Blot, Expressing, Transmission Assay, Immunofluorescence, Fluorescence, shRNA, Negative Control, Over Expression, Plasmid Preparation

Journal: CytoJournal

Article Title: SOX2 promotes the progression of hepatocellular carcinoma by targeting FOXJ3 and activating the PI3K/AKT pathway to regulate autophagy and mitophagy

doi: 10.25259/Cytojournal_164_2025

Figure Lengend Snippet: SOX2 regulates the expression of FOXJ3 in HCC. (a-c) qRT-PCR and Western blot analysis of FOXJ3 mRNA and protein expression levels in Huh7 cells following SOX2 knockdown and overexpression. n = 6; ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, HCC: Hepatocellular carcinoma, FOXJ3: Forkhead box J3, qRT-PCR: Quantitative reverse transcription polymerase chain reaction.

Article Snippet: Human normal liver epithelial cells (THLE-2, CL-0833) and HCC cells (Huh7, CL-0120) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Knockdown, Over Expression, Reverse Transcription, Polymerase Chain Reaction

Journal: CytoJournal

Article Title: SOX2 promotes the progression of hepatocellular carcinoma by targeting FOXJ3 and activating the PI3K/AKT pathway to regulate autophagy and mitophagy

doi: 10.25259/Cytojournal_164_2025

Figure Lengend Snippet: SOX2 regulates autophagy and mitophagy in HCC cells by regulating FOXJ3. (a-c) Western blot analysis of the effects of SOX2 and FOXJ3 on the expression levels of autophagy-related proteins LC3II, LC3I, and p62. (d) Transmission electron microscopic observation of autophagosome formation in HCC cells under SOX2 and FOXJ3 modulation. Scale bar: 50 nm. (e-g) Western blot analysis of the effects of SOX2 and FOXJ3 on mitophagy-related proteins PINK1, phosphorylated COX4 (p-COX4), and COX4. (h and i) Immunofluorescence assay assessing the colocalization of mitochondria and lysosomes upon SOX2 and FOXJ3 expression. (h) Magnification = ×200, Scale bar: 50 μm. n = 6; ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, HCC: Hepatocellular carcinoma, FOXJ3: Forkhead box J3.

Article Snippet: Human normal liver epithelial cells (THLE-2, CL-0833) and HCC cells (Huh7, CL-0120) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Western Blot, Expressing, Transmission Assay, Immunofluorescence